Before you start

So, you want to add a new pipeline to nf-core - brilliant! Before you start typing, check that you're happy with the following points:

Join the community

At its heart, nf-core is a community - to add a pipeline you need to be part of that community! Please request to join the nf-core GitHub organisation) and introduce yourself on Slack or the mailing list.

It's good to introduce your idea early on so that it can be discussed before you spend lots of time coding.

Create a pipeline from the template

The guidelines for nf-core pipelines are pretty strict, but if you start your pipeline by using the nf-core template (nf-core create - see the docs) then your life will be much easier. This tool does lots of things for you: it gives you the correct file structure and boiler plate code and also sets up the required git infrastructure for you to keep your pipeline in sync in the future.

Even if you already have a working pipeline, it may be easier in the long run to use this this template and copy over your code in the relevant places.

If you really don't want to use the template it should possible to work without it. Please see the manual synchronisation documentation.

Note that workflow names should be all lower-case and contain no punctuation. This is to allow consistent names between platforms (eg. GitHub + Docker Hub).

Push to GitHub

Create a repository on GitHub for your new pipeline under your personal account. Make sure not to initialise it with README file - you just want an empty repository.

Once created, copy the URL and add this as a remote to your local git repository and push your code:

# Add a remote called 'origin' - this is the default name for a primary remote
git remote add origin
# Commit any new code changes
git add .
git commit -m "Starting to build my pipeline"
# Push to GitHub
git push

NB: We hope to automate these steps with the nf-core create command soon.

Set up Travis and Docker Hub

The nf-core pipelines use two additional services that link to GitHub: Travis and Dockerhub. You'll want to set both of these up for your new pipeline to enable automated testing (Travis) and automatic Docker image builds (Docker Hub).

To enable Travis, go to and log in with your GitHub credentials. Add a new GitHub repository and select your new pipeline. The repository should already contain the required .travis.yml file, so the next time you push a commit the tests will be automatically triggered.

The process for Docker Hub is similar, though made a little complicated by the number of different docker tools (docker hub, docker cloud, docker swarm etc.). Docker Hub and Docker Cloud use the same back-end, so it doesn't really matter which website you use. Docker Cloud has a nicer interface though, so we recommend that.

  1. Go to and create an account
  2. Create a new repository for your workflow
  3. Set your repository to be automatically built from a GitHub repository and link it to your workflow
  4. Configure the repo to automatically build whenever you push a new commit to your GitHub repo

Whilst developing your pipeline on your local fork you will need to create automated builds for two docker images with source set to master - one with the dev tag and the other with the latest tag. The former will be required for Travis and the latter will be pulled when executing the pipeline locally.

NB: The default name (nfcore/<PIPELINE_NAME>) for the Docker image will need to be replaced in the pipeline template i.e. container variable in nextflow.config and docker commands in .travis.yml. These will need to be changed back to the defaults before you fork the pipeline to nf-core.

Work on your pipeline

Ok, now you're all set with your own personal nf-core pipeline! You can now start writing code for real. Remember to keep running the nf-core lint command (see docs) to make sure that your workflow passes all of the nf-core tests. The automated tests on Travis also run this so you should get an email if something breaks.

Add some test data

Whilst the linting tests are good, they're not sufficient by themselves. It's also good to get Travis to actually run your pipeline on a minimal dataset. Currently, we don't usually check the results that are produced, but it often catches syntax errors and other serious problems that cause nextflow to exit with an error.

Putting the test data on GitHub

To avoid bloating the workflow, we don't keep test data in the same repository as nf-core workflows. Instead, we use the dedicated nf-core/test-datasets repository.

To set this up, make a fork of that repo to your personal account. Clone the repository and check out a new branch for your workflow:

git clone
cd test-datasets
git checkout -b MY_WORKFLOW

Now add your test data files - note that they must be very small. GitHub has quite a low file size limit, and the Travis jobs will time out with anything that's not tiny. We typically use PhiX / Yeast / part of a chromosome as a reference and aggressively subsampled input data.

Once added, push these new files to GitHub:

git add .
git commit -m "Added test data for MY_WORKFLOW"
git push --set-upstream origin MY_WORKFLOW

Finally, make a pull-request against the main nf-core/test-datasets repository with your files. You want this repo to also use a branch with the name of your workflow, so first go to the repository GitHub web page and create this new branch using the UI there. Once created, you can open a pull request and select this as the target branch.

If in doubt, ask for help! (Slack or mailing list)

Setting up a test workflow

Now that your test data is hosted on the web, you can set up a test config profile in your workflow that points to it. In fact, the test profile should already exist if you've used the template. Switch out the example URLs for the ones you added (view the files on GitHub and click 'Raw' to get the URL).

Add any other required parameters so that running the pipeline runs with as few extra flags as possible. Note that the test profile can be combined with other profiles such as docker or conda, so your config should not specify a hardware environment.

Have a go at running the pipeline and see if it works:

nextflow run MY_WORKFLOW -profile test,docker

Note that if you do need to adjust this nextflow run command, you'll need to update it in the .travis.yml config file too.

Adding your pipeline to the nf-core organisation

Ok, so you're essentially finished. Your pipeline is written, the tests pass and you're ready to add your workflow to nf-core.

First, fork your workflow repository to the nf-core GitHub organisation by clicking 'Fork' at the top of the GitHub webpage. If you don't see nf-core as an option, please ask one of the nf-core administrators to do this for you.

Once forked, the nf-core website will automatically update to list your new pipeline.

Setting up Travis and Docker Hub

Just as with your own fork, Travis and Docker Hub need to be set up for the main nf-core fork. If in doubt, please ask an nf-core administrator to help you with this.

Repository setup

You or someone else should set up the new repository with the following:

Core pipeline review

Ok - now the tough bit - does your workflow stand up to the scrutiny of the nf-core team?! Not to worry, we're a friendly bunch. Let us know about the new pipeline, when you're ready we will create a fake pull-request against the first commit in the pipeline. This gives the PR review interface showing all code that you've written. We will go through everything and request and changes that we think are necessary until you're good to go.

Differences to your own fork

The main difference when working with the main nf-core fork of your workflow is that tests for pull-requests against the master branch will fail. This is because the master branch should only ever contain code from the last release. Instead, use the dev branch for new work and always make pull-requests against that. Then the tests should pass.

Making a release

When the code is stable and ready for a release, make a pull-request from the dev branch to master on the nf-core fork. This is a special case and the tests should pass. Once they do, merge the PR and create a new release on GitHub.

The nf-core website and helper tools will automatically detect new releases and update accordingly.

That's it, you're finished! Congratulations!