UNDER CONSTRUCTION: Hybrid genome assembly pipeline using a combination of long and short sequencing reads
The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible.
The nf-core/denovohybrid pipeline comes with documentation about the pipeline, found in the
- Pipeline configuration
- Running the pipeline
- Output and how to interpret the results
The Denovohybrid pipeline allows the generation of high quality genome assemblies using a combination of long read data (Oxford Nanopore Technologies) and short read data (Illumina Technologies). It includes read preprocessing and quality control, a choice of different assembly methods and quality control of the resulting assemblies. Depending on the assembly method it can be used for Bacteria (using Unicycler) or Eukaryotic samples (using miniasm or wtdbg2). When no short read data is provided, this pipeline automatically creates a long read only assembly.
nf-core/denovohybrid was originally written by Caspar Groß.