nf-core/diseasemodulediscovery
A pipeline for network-based disease module identification.
Introduction
nf-core/diseasemodulediscovery is a bioinformatics pipeline for network medicine hypothesis generation, designed for identifying active/disease modules. Developed and maintained by the RePo4EU consortium, it aims to characterize the molecular mechanisms of diseases by analyzing the local neighborhood of disease-associated genes or proteins (seeds) within the interactome. This approach can help identify potential drug targets for drug repurposing.
Running the pipeline
The typical command for running the pipeline is as follows:
nextflow run run nf-core/diseasemodulediscovery \
-profile docker \
--seeds ./seeds.txt \
--network ./ppi.csv \
--id_space entrez \
--outdir ./results
This will launch the pipeline with the docker
configuration profile. See below for more information about profiles.
--seeds
has to point to a text file with seed genes or proteins, without a header and one line per entry.
--network
has to point to file containing a background network. This can be a CSV edge list (without header), or a .gt, .graphml, or .dot file. Alternatively, you can choose one of the available background networks (see below).
--id_space
has to indicate the ID space your genes or proteins are using. For genes Entrez IDs, Ensembl IDs, and HGNC Symbols are supported. For proteins UniProt-AC IDs are supported.
Note that the pipeline will create the following files in your working directory:
work # Directory containing the nextflow working files
<OUTDIR> # Finished results in specified location (defined with --outdir)
.nextflow_log # Log file from Nextflow
# Other nextflow hidden files, eg. history of pipeline runs and old logs.
If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file.
Pipeline settings can be provided in a yaml
or json
file via -params-file <file>
.
Do not use -c <file>
to specify parameters as this will result in errors. Custom config files specified with -c
must only be used for tuning process resource specifications, other infrastructural tweaks (such as output directories), or module arguments (args).
The above pipeline run specified with a params file in yaml format:
nextflow run nf-core/diseasemodulediscovery -profile docker -params-file params.yaml
with:
input: './samplesheet.csv'
outdir: './results/'
<...>
You can also generate such YAML
/JSON
files via nf-core/launch.
Available networks
Instead of providing your own network file, you can choose from a variety of already prepared human PPI networks by specifying a key word instead of a file path. The available networks are:
Key word | Version | Nodes* | Edges* | Description |
---|---|---|---|---|
string_min900 | v12.0 | 11,971 | 93,559 | Human PPI network obtained from STRING, including highest-confidence physical and functional interactions with a score greater than 0.9. |
string_min700 | v12.0 | 15,788 | 224,045 | Human PPI network obtained from STRING, including high-confidence physical and functional interactions with a score greater than 0.7. |
string_physical_min900 | v12.0 | 7,722 | 34,141 | Human PPI network obtained from STRING, including highest-confidence physical interactions with a score greater than 0.9. |
string_physical_min700 | v12.0 | 10,465 | 78,878 | Human PPI network obtained from STRING, including highest-confidence physical interactions with a score greater than 0.7. |
biogrid | 4.4.242 | 18,101 | 865,553 | Human PPI network obtained from BioGRID. |
hippie_high_confidence | v2.3 | 13,246 | 112,202 | Human PPI network obtained from HIPPIE, including only high-confidence interactions with a score greater than 0.73. |
hippie_medium_confidence | v2.3 | 16,613 | 637,499 | Human PPI network obtained from HIPPIE, including only medium-confidence interactions with a score greater than 0.63. |
iid | 18.03.2025 | 19,598 | 1,202,716 | Human PPI network obtained from IID. |
nedrex | 18.03.2025 | 18,718 | 935,139 | Human PPI network queried from NeDRexDB, including only experimentally validated interactions. |
nedrex_high_confidence | 18.03.2025 | 12,827 | 95,944 | Human PPI network queried from NeDRexDB, including only experimentally validated high-confidence interactions with a score greater than 13.5. |
*The numbers of nodes and edges refer to the UniProt ID versions.
Usage example:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf \
-profile docker \
--seeds ./seeds.txt \
--network string_min900 \
--id_space uniprot \
--outdir ./results
This will automatically download and apply the string_min900
network.
The prepared networks are hosted via Zenodo.
All networks are available for all supported ID spaces.
The correct ID space is automatically determined based on the --id_space
parameter.
For details on the network preparation procedure (including ID mapping, links to original sources, and network sizes) see the corresponding git repo.
Multiple seed files or networks
You can also use the --seeds
and --network
parameters to define multiple files as comma-separated lists:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf \
-profile <docker/singularity> \
--seeds <SEED_FILE_1,SEED_FILE_2,...> \
--network <NETWORK_FILE_1,NETWORK_FILE_2,...> \
--outdir <OUTDIR>
If multiple files are provided for both options, the pipeline will run for every possible combination of seeds and network files.
In case you are only interested in specific combinations, seeds-network pairs can be specified via a CSV samplesheet:
samplesheet.csv
:
seeds,network
seed_file_1.csv,network_1.csv
seed_file_2.csv,network_2.csv
seed_file_2.csv,network_1.csv
Each row defines a seeds-network pair.
You can run the pipeline with a samplesheet using the --input
parameter instead of --seeds
and --network
:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf \
-profile <docker/singularity> \
--input samplesheet.csv \
--outdir <OUTDIR>
Skipping steps
Most pipeline steps can be skipped using --skip_<PIPELINE_STEP>
. E.g., if you are only interested in module discovery, you can skip the annotation and evaluation steps using:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf \
-profile <docker/singularity> \
--input samplesheet.csv \
--outdir <OUTDIR> \
--skip_annotation \
--skip_evaluation
You can then later continue the pipeline (including evaluation and annotation) using the -resume
option:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf \
-profile <docker/singularity> \
--input samplesheet.csv \
--outdir <OUTDIR> \
-resume
To see the full list of skipping options, please run:
nextflow run <PATH_TO_REPO>/modulediscovery/main.nf --help
Updating the pipeline
When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you’re running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline:
nextflow pull nf-core/diseasemodulediscovery
Reproducibility
It is a good idea to specify the pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you’ll be running the same version of the pipeline, even if there have been changes to the code since.
First, go to the nf-core/diseasemodulediscovery releases page and find the latest pipeline version - numeric only (eg. 1.3.1
). Then specify this when running the pipeline with -r
(one hyphen) - eg. -r 1.3.1
. Of course, you can switch to another version by changing the number after the -r
flag.
This version number will be logged in reports when you run the pipeline, so that you’ll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports.
To further assist in reproducibility, you can use share and reuse parameter files to repeat pipeline runs with the same settings without having to write out a command with every single parameter.
If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles.
Core Nextflow arguments
These options are part of Nextflow and use a single hyphen (pipeline parameters use a double-hyphen)
-profile
Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments.
Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below.
We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.
The pipeline also dynamically loads configurations from https://github.com/nf-core/configs when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is supported, please see the nf-core/configs documentation.
Note that multiple profiles can be loaded, for example: -profile test,docker
- the order of arguments is important!
They are loaded in sequence, so later profiles can overwrite earlier profiles.
If -profile
is not specified, the pipeline will run locally and expect all software to be installed and available on the PATH
. This is not recommended, since it can lead to different results on different machines dependent on the computer environment.
test
- A profile with a complete configuration for automated testing
- Includes links to test data so needs no other parameters
docker
- A generic configuration profile to be used with Docker
singularity
- A generic configuration profile to be used with Singularity
podman
- A generic configuration profile to be used with Podman
shifter
- A generic configuration profile to be used with Shifter
charliecloud
- A generic configuration profile to be used with Charliecloud
apptainer
- A generic configuration profile to be used with Apptainer
wave
- A generic configuration profile to enable Wave containers. Use together with one of the above (requires Nextflow
24.03.0-edge
or later).
- A generic configuration profile to enable Wave containers. Use together with one of the above (requires Nextflow
conda
- A generic configuration profile to be used with Conda. Please only use Conda as a last resort i.e. when it’s not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer.
-resume
Specify this when restarting a pipeline. Nextflow will use cached results from any pipeline steps where the inputs are the same, continuing from where it got to previously. For input to be considered the same, not only the names must be identical but the files’ contents as well. For more info about this parameter, see this blog post.
You can also supply a run name to resume a specific run: -resume [run-name]
. Use the nextflow log
command to show previous run names.
-c
Specify the path to a specific config file (this is a core Nextflow command). See the nf-core website documentation for more information.
Custom configuration
Resource requests
Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the pipeline steps, if the job exits with any of the error codes specified here it will automatically be resubmitted with higher resources request (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped.
To change the resource requests, please see the max resources and tuning workflow resources section of the nf-core website.
Custom Containers
In some cases, you may wish to change the container or conda environment used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the biocontainers or bioconda projects. However, in some cases the pipeline specified version maybe out of date.
To use a different container from the default container or conda environment specified in a pipeline, please see the updating tool versions section of the nf-core website.
Custom Tool Arguments
A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default.
To learn how to provide additional arguments to a particular tool of the pipeline, please see the customising tool arguments section of the nf-core website.
nf-core/configs
In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the nf-core/configs
git repository. Before you do this please can you test that the config file works with your pipeline of choice using the -c
parameter. You can then create a pull request to the nf-core/configs
repository with the addition of your config file, associated documentation file (see examples in nf-core/configs/docs
), and amending nfcore_custom.config
to include your custom profile.
See the main Nextflow documentation for more information about creating your own configuration files.
If you have any questions or issues please send us a message on Slack on the #configs
channel.
Running in the background
Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished.
The Nextflow -bg
flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file.
Alternatively, you can use screen
/ tmux
or similar tool to create a detached session which you can log back into at a later time.
Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs).
Nextflow memory requirements
In some cases, the Nextflow Java virtual machines can start to request a large amount of memory.
We recommend adding the following line to your environment to limit this (typically in ~/.bashrc
or ~./bash_profile
):
NXF_OPTS='-Xms1g -Xmx4g'