Description

Run the alignment/variant-call/consensus logic of the artic pipeline

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

fastq:file

FastQ file containing reads

*.{fastq.gz}

fast5_dir:directory

Directory containing MinION FAST5 files

*

sequencing_summary:file

Path to Guppy sequencing summary

*.{txt}

"primer-schemes/${scheme}/V${scheme_version}/${scheme}.reference.fasta":file

Scheme reference file

"primer-schemes/${scheme}/V${scheme_version}/${scheme}.scheme.bed":file

Scheme BED file

medaka_model_file:file

Medaka model file to use (if option —medaka is set)

*.hdf5

medaka_model_string:string

Medaka model string to use (if option —medaka is set)

*

scheme:string

Name of the primer scheme

scheme_version:string

Version of the primer scheme

Output

name:type
description
pattern

results

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.*:file

Aggregated FastQ files

*.fastq.gz

bam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.sorted.bam:file

BAM file

*.{sorted.bam}

bai

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.sorted.bam.bai:file

BAM index file

*.{sorted.bai}

bam_trimmed

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.trimmed.rg.sorted.bam:file

BAM file with the primers left on

*.{trimmed.rg.sorted.bam}

bai_trimmed

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.trimmed.rg.sorted.bam.bai:file

BAM index file of bam_trimmed

*.{sorted.bai}

bam_primertrimmed

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.primertrimmed.rg.sorted.bam:file

BAM containing reads after primer-binding site trimming

*.{trimmed.rg.sorted.bam}

bai_primertrimmed

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.primertrimmed.rg.sorted.bam.bai:file

BAM index file of bam_primertrimmed

*.{primertrimmed.rg.sorted.bam.bai}

fasta

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.consensus.fasta:file

FAST file with consensus sequence

*.{consensus.fasta}

vcf

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.pass.vcf.gz:file

VCF file containing detected variants passing quality filter

*.{pass.vcf.gz}

tbi

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}.pass.vcf.gz.tbi:file

VCF index

*.{pass.vcf.gz.tbi}

json

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.json:file

JSON file for MultiQC

*.json

versions

versions.yml:file

File containing software versions

versions.yml

Tools

artic
MIT

ARTIC pipeline - a bioinformatics pipeline for working with virus sequencing data sequenced with nanopore