Description

generate VCF file from a BAM file using various calling methods

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bam{:bash}

:file

A BAM/ file

*.bam

bai{:bash}

:file

The BAI file for the input BAM file

*.bai

pmd{:bash}

:file

Optional PMD file from atlas pmd function in text format

*.txt

recal{:bash}

:file

Optional recalibration file from atlas recal function in text format

*.txt

fasta{:bash}

:file

The reference FASTA file used to generate the BAM file

*.{fasta,fna,fa}

fai{:bash}

:file

The index of the FASTA file used for to generate the BAM file

*.fai

known_alleles{:bash}

:file

Optional tab separated text file containing 1-based list of known alleles. See atlas call documentation.

*.{txt.tsv}

method{:bash}

:string

Which variant calling algorithm to use. Some may require additional parameters supplied via ext.args. Check atlas documentation.

MLE|Bayesian|allelePresence|randomBase|majorityBase

Output

name:type
description
pattern

vcf{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.vcf.gz{:bash}

:file

VCF file with variant calls

*.vcf.gz

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

atlas
GPL v3

ATLAS, a suite of methods to accurately genotype and estimate genetic diversity