Description

Estimate the post-mortem damage patterns of DNA

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

bam:file

Single input BAM file

*.bam

bai:file

The BAI file for the input BAM file

*.bai

pool_rg_txt:file

Optional. Provide the names of read groups that should be merged for PMD estimation. All read groups that should be pooled listed on one line, separated by any white space. Other read groups will be recalibrated separately.

*.txt

fasta:file

The reference genome provided as FASTA file

*.fasta

fai:file

The FAI file for the reference genome FASTA file

*.fai

Output

name:type
description
pattern

empiric

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_PMD_input_Empiric.txt:file

A list of pmd patterns estimated with the empirical method for each readgroup

*_PMD_input_Empiric.txt

exponential

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_PMD_input_Exponential.txt:file

A list of pmd patterns estimated with the exponential method for each readgroup

*_PMD_input_Exponential.txt

counts

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_PMD_Table_counts.txt:file

The counts of all possible transitions for each read position (or up to a certain position, see specific command length)

*_PMD_Table_counts.txt

table

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_PMD_Table.txt:file

For all possible transitions the ratio of the transition counts, which are taken from the _counts.txt table, over the total amount of the base that was mutated, for each position and readgroup

*_PMD_Table.txt

versions

versions.yml:file

File containing software versions

versions.yml

Tools

atlas
GPL v3

ATLAS, a suite of methods to accurately genotype and estimate genetic diversity