Description

Demultiplex Element Biosciences bases files

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

run_manifest:file

RunManifest file

*.{csv}

run_dir:directory

Input run directory containing optionally containing a RunManifest.json if run_manifest is not supplied

Output

name:type
description
pattern

sample_fastq

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/Samples/**/*_R*.fastq.gz:file

Demultiplexed sample FASTQ files

output/Samples/*/*_R*.fastq.gz

sample_json

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/Samples/**/*_stats.json:file

Demultiplexed sample stats

output/Samples/*/*.json

qc_report

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/*.html:file

QC HTML report

output/*.html

run_stats

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/RunStats.json:file

QC HTML report

output/*.html

generated_run_manifest

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/RunManifest.json:file

Updated Run Manifest JSON from the run_manifest csv

output/RunManifest.json

metrics

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/Metrics.csv:file

Sample metrics

output/Metrics.csv

unassigned

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

output/UnassignedSequences.csv:file

Unassigned Sequences

output/UnassignedSequences.csv

versions

versions.yml:file

File containing software versions

versions.yml

Tools

bases2fastq
http://go.elembio.link/eula

Demultiplexes sequencing data and converts base calls into FASTQ files for secondary analysis