Description

Split sequencing reads by mapping them to multiple references simultaneously

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

reads:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

index:directory

Directory to place generated index

*

primary_ref:file

Path to the primary reference

*

other_ref_names:list

List of other reference ids apart from the primary

other_ref_paths:list

Path to other references paths corresponding to “other_ref_names”

only_build_index:string

true = only build index; false = mapping

Output

name:type
description
pattern

index

bbsplit:directory

Directory with index files

bbsplit

primary_fastq

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*primary*fastq.gz:file

Output reads that map to the primary reference

*primary*fastq.gz

all_fastq

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*fastq.gz:file

All reads mapping to any of the references

*fastq.gz

stats

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*txt:file

Tab-delimited text file containing mapping statistics

*.txt

log

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.log:file

Log file

*.log

versions

versions.yml:file

File containing software versions

versions.yml

Tools

bbmap
UC-LBL license (see package)

BBMap is a short read aligner, as well as various other bioinformatic tools.