Description

Aligns single- or paired-end reads from bisulfite-converted libraries to a reference genome using Biscuit.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

fasta

:file

Input genome fasta file

meta3

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

index

:directory

Directory containing biscuit genome index

Output

name:type
description
pattern

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bam

:file

Output BAM file containing read alignments

*.{bam}

bai

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bai

:file

Output BAM index

*.{bai}

versions_biscuit

${task.process}

:string

The name of the process

biscuit

:string

The name of the tool

biscuit version |& sed '1!d; s/^.*BISCUIT Version: //'

:eval

The expression to obtain the version of the tool

versions_samtools

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools --version |& sed '1!d; s/^.*samtools //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

biscuit

:string

The name of the tool

biscuit version |& sed '1!d; s/^.*BISCUIT Version: //'

:eval

The expression to obtain the version of the tool

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools --version |& sed '1!d; s/^.*samtools //'

:eval

The expression to obtain the version of the tool

Tools

biscuit
MIT

A utility for analyzing sodium bisulfite conversion-based DNA methylation/modification data