Description

Summarizes read-level methylation (and optionally SNV) information from a Biscuit BAM file in a standard-compliant BED format.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bam

:file

BAM file contained mapped reads

meta2

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bai

:file

BAM file index

meta3

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

snp_bed

:file

BED file containing SNP information (optional)

meta4

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

fasta

:file

Input genome fasta file

meta5

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

index

:directory

Directory containing biscuit genome index

Output

name:type
description
pattern

bed

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bed.gz

:file

Gzipped BED file with methylation (and optionally SNV) information

*.{epiread.bed.gz}

versions_biscuit

${task.process}

:string

The name of the process

biscuit

:string

The name of the tool

biscuit version |& sed '1!d; s/^.*BISCUIT Version: //'

:eval

The expression to obtain the version of the tool

versions_samtools

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools --version |& sed '1!d; s/^.*samtools //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

biscuit

:string

The name of the tool

biscuit version |& sed '1!d; s/^.*BISCUIT Version: //'

:eval

The expression to obtain the version of the tool

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools --version |& sed '1!d; s/^.*samtools //'

:eval

The expression to obtain the version of the tool

Tools

biscuit
MIT

A utility for analyzing sodium bisulfite conversion-based DNA methylation/modification data