Description

Performs fastq alignment to a fasta reference using BWA

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

reads:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2:map

Groovy Map containing reference/index information e.g. [ id:‘test’ ]

index:file

BWA genome index files

Directory containing BWA index *.{0132,amb,ann,bwt.2bit.64,pac}

meta3:map

Groovy Map containing reference information e.g. [ id:‘genome’ ]

fasta:file

Reference genome in FASTA format

*.{fa,fasta,fna}

sort_bam:boolean

use samtools sort (true) or samtools view (false)

true or false

Output

name:type
description
pattern

sam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.sam:file

Output SAM file containing read alignments

*.{sam}

bam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.bam:file

Output BAM file containing read alignments

*.{bam}

cram

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.cram:file

Output CRAM file containing read alignments

*.{cram}

crai

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.crai:file

Index file for CRAM file

*.{crai}

csi

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.csi:file

Index file for BAM file

*.{csi}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

bwa
MIT

BWA-mem2 is a software package for mapping DNA sequences against a large reference genome, such as the human genome.