Description

DAS Tool binning step.

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

contigs (file)

fasta file

*.{fa.gz,fas.gz,fasta.gz}

bins (file)

FastaToContig2Bin tabular file generated with dastool/fastatocontig2bin

*.tsv

proteins (file)

Predicted proteins in prodigal fasta format (>scaffoldID_geneNo)

*.{fa.gz,fas.gz,fasta.gz}

db_directory (file)

(optional) Directory of single copy gene database.

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

version (file)

File containing software version

versions.yml

log (file)

Log file of the run

*.log

summary (file)

Summary of output bins including quality and completeness estimates

*summary.txt

contig2bin (file)

Scaffolds to bin file of output bins

*.contig2bin.txt

eval (file)

Quality and completeness estimates of input bin sets

*.eval

bins (file)

Final refined bins in fasta format

*.fa

pdfs (file)

Plots showing the amount of high quality bins and score distribution of bins per method

*.pdf

fasta_proteins (file)

Output from prodigal if not already supplied

*.proteins.faa

fasta_archaea_scg (file)

Results of archaeal single-copy-gene prediction

*.archaea.scg

fasta_bacteria_scg (file)

Results of bacterial single-copy-gene prediction

*.bacteria.scg

b6 (file)

Results in b6 format

*.b6

seqlength (file)

Summary of contig lengths

*.seqlength
included in
mag
authors
Github user maxibor Github user jfy133
get in touch
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Tools

dastool
BSD

DAS Tool is an automated method that integrates the results of a flexible number of binning algorithms to calculate an optimized, non-redundant set of bins from a single assembly.

github.com/cmks/DAS_Tool 10.1038/s41564-018-0171-1