Description

This tool takes an alignment of reads or fragments as input (BAM file) and generates a coverage track (bigWig or bedGraph) as output.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

input

:file

BAM/CRAM file

*.{bam,cram}

input_index

:file

BAM/CRAM index file

*.{bai,crai}

fasta

:file

Reference file the CRAM file was created with (required with CRAM input)

*.{fasta,fa}

fasta_fai

:file

Index of the reference file (optional, but recommended)

*.{fai}

meta2

:map

Groovy Map containing blacklist metadata e.g. [ id:‘blacklist’ ]

blacklist

:file

BED/GTF file containing regions to exclude from analysis

*.{bed,gtf}

Output

name:type
description
pattern

bigwig

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bigWig

:file

BigWig file

*.bigWig

bedgraph

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bedgraph

:file

Bedgraph file

*.bedgraph

versions_deeptools

${task.process}

:string

The name of the process

deeptools

:string

The name of the tool

bamCoverage --version | sed "s/bamCoverage //g"

:eval

The expression to obtain the version of the tool

versions_samtools

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools version | sed '1!d;s/.* //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

deeptools

:string

The name of the tool

bamCoverage --version | sed "s/bamCoverage //g"

:eval

The expression to obtain the version of the tool

${task.process}

:string

The name of the process

samtools

:string

The name of the tool

samtools version | sed '1!d;s/.* //'

:eval

The expression to obtain the version of the tool

Tools

deeptools
GPL v3

A set of user-friendly tools for normalization and visualization of deep-sequencing data