Description

Calls consensus sequences from reads with the same unique molecular tag.

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

, collapse
]

grouped_bam (file)

The input SAM or BAM file, grouped by UMIs

*.{bam,sam}

min_reads (integer)

Minimum number of original reads to build each consensus read.

min_baseq (integer)

Ignore bases in raw reads that have Q below this value.

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

bam (file)

Output SAM or BAM file to write consensus reads.

*.{bam,sam}

versions (file)

File containing software versions

versions.yml

Tools

fgbio
MIT

Tools for working with genomic and high throughput sequencing data.