Description

Using the fgbio tools, converts FASTQ files sequenced into unaligned BAM or CRAM files possibly moving the UMI barcode into the RX field of the reads

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

reads:file

pair of reads to be converted into BAM file

*.{fastq.gz}

Output

name:type
description
pattern

bam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.bam:file

Unaligned, unsorted BAM file

*.{bam}

cram

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.cram:file

Unaligned, unsorted CRAM file

*.{cram}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

fgbio
MIT

A set of tools for working with genomic and high throughput sequencing data, including UMIs