Description

Splits reads that contain Ns in their cigar string

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’]

bam{:bash}

:list

BAM/SAM/CRAM file containing reads

*.{bam,sam,cram}

bai{:bash}

:list

BAI/SAI/CRAI index file (optional)

*.{bai,sai,crai}

intervals{:bash}

:file

Bed file with the genomic regions included in the library (optional)

meta2{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘reference’ ]

fasta{:bash}

:file

The reference fasta file

*.fasta

meta3{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘reference’ ]

fai{:bash}

:file

Index of reference fasta file

*.fasta.fai

meta4{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘reference’ ]

dict{:bash}

:file

GATK sequence dictionary

*.dict

Output

name:type
description
pattern

bam{:bash}

meta{:bash}

:file

Output file with split reads (BAM/SAM/CRAM)

*.{bam,sam,cram}

*.bam{:bash}

:file

Output file with split reads (BAM/SAM/CRAM)

*.{bam,sam,cram}

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

gatk4
Apache-2.0

Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size.