Description

Helper script, remove remaining polyA sequences from Full Length Non Chimeric reads (Pacbio isoseq3)

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

fasta:file

Full Length Non Chimeric reads in fasta format

*.{fa,fasta}

Output

name:type
description
pattern

fasta

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_tama.fa.gz:file

The Full Length Non Chimeric reads clened from remaining polyA tails. The sequences are in FASTA format compressed with gzip.

*_tama.fa.gz

report

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_tama_polya_flnc_report.txt.gz:file

A text file describing the number of polyA tails removed and their length. Compressed with gzip.

*_tama_polya_flnc_report.txt.gz

tails

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_tama_tails.fa.gz:file

A gzip compressed FASTA file of trimmed polyA tails.

*_tama_tails.fa.gz

versions

versions.yml:file

File containing software versions

versions.yml

Tools