Demultiplex samples based on data from cell hashing.
meta{:bash}
:map
Groovy Map containing sample information e.g. [ id:'sample1' ]
[ id:'sample1' ]
seurat_object{:bash}
:file
A .rds file containing the seurat object. Assumes that the hash tag oligo (HTO) data has been added and normalized.
.rds
assay{:bash}
:string
Name of the Hashtag assay, usually called “HTO” by default. Use the custom name if the assay has been named differently.
params{:bash}
*_params_htodemux.csv{:bash}
The used parameters to call HTODemux in the R-Script.
params_htodemux.csv
assignment{:bash}
*_assignment_htodemux.csv{:bash}
Assignment results.
assignment_htodemux.csv
classification{:bash}
*_classification_htodemux.csv{:bash}
Classification results.
classification_htodemux.csv
rds{:bash}
*_htodemux.rds{:bash}
SeuratObject saved as RDS.
htodemux.rds
versions{:bash}
versions.yml{:bash}
File containing software versions
versions.yml
HTODemux is the demultiplexing module of Seurat, which demultiplex samples based on data from cell hashing.
