Description

Ilastik is a tool that utilizes machine learning algorithms to classify pixels, segment, track and count cells in images. Ilastik contains a graphical user interface to interactively label pixels. However, this nextflow module will implement the —headless mode, to apply pixel classification using a pre-trained .ilp file on an input image.

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

h5:file

h5 file containing image stack to classify file

*.{h5,hdf5}

meta2:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

ilp:file

Trained ilastik .ilp project file

*.{ilp}

meta3:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

probs:file

Probability map for boundary based segmentation

*.{h5,,hdf5}

Output

name:type
description
pattern

out_tiff

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.tiff:file

Multicut segmentation mask output.

*.{tiff}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

ilastik

Ilastik is a user friendly tool that enables pixel classification, segmentation and analysis.