Description

Remove polyA tail and artificial concatemers

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test ]

bam:file

BAM file, cleaned ccs generated by lima

*.bam

primers:file

fasta file of primers

*.fasta

Output

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

bam:file

Set of complete reads (with polyA tail), where the polyA has been trimmed

*.bam

pbi:file

Pacbio index file from polyA trimmed reads

*.pbi

consensusreadset:file

Metadata about read library

*.xml

summary:file

json file describing number of full length reads, full length non chimeric reads and full length non chimeric polyA reads

*.json

report:file

Metadata about primer and polyA detection (primers/polyA/insert length, strand, primer name)

*.csv

versions:file

File containing software versions

versions.yml

Tools

isoseq3
BSD-3-Clause-Clear

IsoSeq3 - Scalable De Novo Isoform Discovery