Extract UMI and cell barcodes
meta
:map
Groovy Map containing sample information e.g. [ id:'sample1' ]
[ id:'sample1' ]
bam
:file
BAM file, one full-length CCS file generated by lima
*.5p--3p.bam
design
:string
Barcoding design. Specifies which bases to use as cell/molecular barcodes.
^(?:\d{1,2}[UBGX]-)+T$|^(?:\d{1,2}[UBGX]-)+T(?:-\d{1,2}[UBGX])+$|^T(?:-\d{1,2}[UBGX])+$
Groovy Map containing sample information e.g. [ id:'sample1']
[ id:'sample1']
*.flt.bam
BAM file with full-length tagged reads
pbi
*.flt.bam.pbi
Pacbio index file of full-length tagged reads
versions_isoseq3
${task.process}
The name of the process
isoseq3
The name of the tool
isoseq tag --version | sed -n '1s/isoseq tag \([0-9.]*\).*/\1/p'
:eval
The expression to obtain the version of the tool
versions
Iso-Seq - Scalable De Novo Isoform Discovery
