Description

Extract UMI and cell barcodes

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:'sample1' ]

bam (file)

BAM file, one full-length CCS file generated by lima

*.5p--3p.bam

design (string)

Barcoding design. Specifies which bases to use as cell/molecular barcodes.

^(?:\d{1,2}[UBGX]-)+T$|^(?:\d{1,2}[UBGX]-)+T(?:-\d{1,2}[UBGX])+$|^T(?:-\d{1,2}[UBGX])+$

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:'sample1']

versions (file)

File containing software versions

versions.yml

bam (file)

BAM file with full-length tagged reads

*.flt.bam

pbi (file)

Pacbio index file of full-length tagged reads

*.flt.bam.pbi