Description

Extract UMI and cell barcodes

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

bam{:bash}

:file

BAM file, one full-length CCS file generated by lima

*.5p--3p.bam

design{:bash}

:string

Barcoding design. Specifies which bases to use as cell/molecular barcodes.

^(?:\d{1,2}[UBGX]-)+T$|^(?:\d{1,2}[UBGX]-)+T(?:-\d{1,2}[UBGX])+$|^T(?:-\d{1,2}[UBGX])+$

Output

name:type
description
pattern

bam{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1']

*.flt.bam{:bash}

:file

BAM file with full-length tagged reads

*.flt.bam

pbi{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1']

*.flt.bam.pbi{:bash}

:file

Pacbio index file of full-length tagged reads

*.flt.bam.pbi

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml