Extract UMI and cell barcodes
meta
:map
Groovy Map containing sample information e.g. [ id:'sample1' ]
[ id:'sample1' ]
bam
:file
BAM file, one full-length CCS file generated by lima
*.5p--3p.bam
design
:string
Barcoding design. Specifies which bases to use as cell/molecular barcodes.
^(?:\d{1,2}[UBGX]-)+T$|^(?:\d{1,2}[UBGX]-)+T(?:-\d{1,2}[UBGX])+$|^T(?:-\d{1,2}[UBGX])+$
Groovy Map containing sample information e.g. [ id:'sample1']
[ id:'sample1']
*.flt.bam
BAM file with full-length tagged reads
pbi
*.flt.bam.pbi
Pacbio index file of full-length tagged reads
versions
versions.yml
File containing software versions
Iso-Seq - Scalable De Novo Isoform Discovery
