Description

Remove polyA tail and artificial concatemers

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test ]

bam:file

BAM file, cleaned ccs generated by lima

*.bam

primers:file

fasta file of primers

*.fasta

Output

name:type
description
pattern

bam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.bam:file

Set of complete reads (with polyA tail), where the polyA has been trimmed

*.bam

pbi

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.bam.pbi:file

Pacbio index file from polyA trimmed reads

*.pbi

consensusreadset

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.consensusreadset.xml:file

Metadata about read library

*.xml

summary

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.filter_summary.report.json:file

json file describing number of full length reads, full length non chimeric reads and full length non chimeric polyA reads

*.json

report

meta:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.report.csv:file

Metadata about primer and polyA detection (primers/polyA/insert length, strand, primer name)

*.csv

versions

versions.yml:file

File containing software versions

versions.yml

Tools

isoseq
BSD-3-Clause-Clear

IsoSeq - Scalable De Novo Isoform Discovery