Remove polyA tail and artificial concatemers
meta:map
Groovy Map containing sample information e.g. [ id:‘test ]
bam:file
BAM file, cleaned ccs generated by lima
*.bam
primers:file
fasta file of primers
*.fasta
bam
Groovy Map containing sample information e.g. [ id:‘test’ ]
*.bam:file
Set of complete reads (with polyA tail), where the polyA has been trimmed
pbi
*.bam.pbi:file
Pacbio index file from polyA trimmed reads
*.pbi
consensusreadset
*.consensusreadset.xml:file
Metadata about read library
*.xml
summary
*.filter_summary.report.json:file
json file describing number of full length reads, full length non chimeric reads and full length non chimeric polyA reads
*.json
report
*.report.csv:file
Metadata about primer and polyA detection (primers/polyA/insert length, strand, primer name)
*.csv
versions
versions.yml:file
File containing software versions
versions.yml
IsoSeq - Scalable De Novo Isoform Discovery