Description

Computes equivalence classes for reads and quantifies abundances

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

*.{fastq,fastq.gz}

meta2

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

index

:file

Kallisto genome index.

*.idx

gtf

:file

Optional gtf file for translation of transcripts into genomic coordinates.

*.gtf

chromosomes

:file

Optional tab separated file with chromosome names and lengths.

*.tsv

fragment_length

:integer

For single-end mode only, the estimated average fragment length.

fragment_length_sd

:integer

For single-end mode only, the estimated standard deviation of the fragment length.

Output

name:type
description
pattern

results

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}

:file

Kallisto output file

json_info

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.run_info.json

:file

JSON file containing information about the run

*.run_info.json

log

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.log

:file

File containing log information from running kallisto quant

*.log.txt

versions_kallisto

${task.process}

:string

The name of the process

kallisto

:string

The name of the tool

kallisto version | sed 's/.*version //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

kallisto

:string

The name of the tool

kallisto version | sed 's/.*version //'

:eval

The expression to obtain the version of the tool

Tools

kallisto
BSD_2_clause

Quantifying abundances of transcripts from RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.