Description

Computes equivalence classes for reads and quantifies abundances

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

reads:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

*.{fastq,fastq.gz}

meta2:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

index:file

Kallisto genome index.

*.idx

gtf:file

Optional gtf file for translation of transcripts into genomic coordinates.

*.gtf

chromosomes:file

Optional tab separated file with chromosome names and lengths.

*.tsv

fragment_length:integer

For single-end mode only, the estimated average fragment length.

fragment_length_sd:integer

For single-end mode only, the estimated standard deviation of the fragment length.

Output

name:type
description
pattern

results

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

${prefix}:file

Kallisto output file

json_info

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.run_info.json:file

JSON file containing information about the run

*.run_info.json

log

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.log:file

File containing log information from running kallisto quant

*.log.txt

versions

versions.yml:file

File containing software versions

versions.yml

Tools

kallisto
BSD_2_clause

Quantifying abundances of transcripts from RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.