Description

lima - The PacBio Barcode Demultiplexer and Primer Remover

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

ccs

:file

A BAM or fasta or fasta.gz or fastq or fastq.gz file of subreads or ccs

*.{bam,fasta,fasta.gz,fastq,fastq.gz}

primers

:file

Fasta file, sequences of primers

*.fasta

Output

name:type
description
pattern

counts

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.counts

:file

A tabulated file of describing pairs of primers

*.counts

report

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.report

:file

A tab-separated file about each ZMW, unfiltered

*.report

summary

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.summary

:file

This file shows how many ZMWs have been filtered, how ZMWs many are same/different, and how many reads have been filtered.

*.summary

versions_lima

${task.process}

:string

The process the versions were collected from

lima

:string

The tool name

lima --version | head -n1 | sed 's/lima //g' | sed 's/ (.\+//g'

:string

The command used to generate the version of the tool

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.bam

:file

A bam file of ccs purged of primers

*.bam

pbi

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.bam.pbi

:file

Pacbio index file of ccs purged of primers

*.bam

fasta

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.{fa,fasta}

:file

A fasta file of ccs purged of primers.

*.fa

fastagz

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.{fa.gz,fasta.gz}

:file

A fasta.gz file of ccs purged of primers.

*.fasta.gz

fastq

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.fastq

:file

A fastq file of ccs purged of primers.

*.fastq

fastqgz

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.fastq.gz

:file

A fastq.gz file of ccs purged of primers.

*.fastq.gz

xml

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.xml

:file

An XML file representing a set of a particular sequence data type such as subreads, references or aligned subreads.

*.xml

json

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.json

:file

A metadata json file

*.json

clips

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.clips

:file

A fasta file of clipped primers

*.clips

guess

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’ ]

*.guess

:file

A second tabulated file of describing pairs of primers (no doc available)

*.guess

Topics

name:type
description
pattern

versions

${task.process}

:string

The process the versions were collected from

lima

:string

The tool name

lima --version | head -n1 | sed 's/lima //g' | sed 's/ (.\+//g'

:string

The command used to generate the version of the tool

Tools

lima
BSD-3-Clause-Clear

lima - The PacBio Barcode Demultiplexer and Primer Remover