Description

maximum-likelihood analysis of gene essentialities computation

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

count_table:file

Count table file. Each line in the table should include sgRNA name (1st column), target gene (2nd column) and read counts in each sample.

*.{txt,tsv}

design_matrix:file

Design matrix describing the samples and conditions

*.{txt,tsv}

Output

name:type
description
pattern

gene_summary

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.gene_summary.txt:file

Gene summary file describing the fitness score and associated p-values.

*.{gene_summary}

sgrna_summary

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.sgrna_summary.txt:file

sgRNA summary file describing the sgRNA and associated gene

*.{gene_summary}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

mageck
BSD

MAGeCK (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout), an algorithm to process, QC, analyze and visualize CRISPR screening data.