Description

MetaPhlAn is a tool for profiling the composition of microbial communities from metagenomic shotgun sequencing data.

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

input:file

Metaphlan can classify the metagenome from a variety of input data types, including FASTQ files (single-end and paired-end), FASTA, bowtie2-produced SAM files (produced from alignments to the MetaPHlAn marker database) and intermediate bowtie2 alignment files (bowtie2out)

*.{fastq.gz, fasta, fasta.gz, sam, bowtie2out.txt}

metaphlan_db_latest:file

Directory containing pre-downloaded and uncompressed MetaPhlAn database downloaded from: http://cmprod1.cibio.unitn.it/biobakery4/metaphlan_databases/. Note that you will also need to specify --index and the database version name (e.g. ‘mpa_vJan21_TOY_CHOCOPhlAnSGB_202103’) in your module.conf ext.args for METAPHLAN_METAPHLAN!

*/

Output

name:type
description
pattern

profile

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*_profile.txt:file

Tab-separated output file of the predicted taxon relative abundances

*.{txt}

biom

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.biom:file

General-use format for representing biological sample by observation contingency tables

*.{biom}

bt2out

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.bowtie2out.txt:file

Intermediate Bowtie2 output produced from mapping the metagenome against the MetaPHlAn marker database ( not compatible with bowtie2out files generated with MetaPhlAn versions below 3 )

*.{bowtie2out.txt}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

metaphlan
MIT License

Identify clades (phyla to species) present in the metagenome obtained from a microbiome sample and their relative abundance