Description

MUSCLE is a program for creating multiple alignments of amino acid or nucleotide sequences. A range of options are provided that give you the choice of optimizing accuracy, speed, or some compromise between the two

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

fasta:file

Input sequences for alignment must be in FASTA format

*.{fasta,fa,fna}

Output

name:type
description
pattern

aligned_fasta

meta:file

Multiple sequence alignment produced in FASTA format

*.{afa}

*.afa:file

Multiple sequence alignment produced in FASTA format

*.{afa}

phyi

meta:file

Multiple sequence alignment produced in PHYLIP interleaved format

*.{phyi}

*.phyi:file

Multiple sequence alignment produced in PHYLIP interleaved format

*.{phyi}

phys

meta:file

Multiple sequence alignment produced in PHYLIP sequential format

*.{phys}

*.phys:file

Multiple sequence alignment produced in PHYLIP sequential format

*.{phys}

clustalw

meta:file

Multiple sequence alignment produced in ClustalW format without base/residue numbering

*.{clw}

*.clw:file

Multiple sequence alignment produced in ClustalW format without base/residue numbering

*.{clw}

html

meta:file

Multiple sequence alignment produced in HTML format

*.{html}

*.html:file

Multiple sequence alignment produced in HTML format

*.{html}

msf

meta:file

GCG Multiple Sequence File (MSF) alignment format (similar to CLUSTALW)

*.{msf}

*.msf:file

GCG Multiple Sequence File (MSF) alignment format (similar to CLUSTALW)

*.{msf}

tree

meta:file

NJ or UPGMA tree in Newick format produced from a multiple sequence alignment

*.{tree}

*.tree:file

NJ or UPGMA tree in Newick format produced from a multiple sequence alignment

*.{tree}

log

*.log:file

Log file of MUSCLE run

*{.log}

versions

versions.yml:file

File containing software versions

versions.yml

Tools

muscle
http://www.drive5.com/muscle/manual/license.html

MUSCLE is a multiple sequence alignment tool with high accuracy and throughput