Description

NVIDIA Clara Parabricks GPU-accelerated alignment, sorting, BQSR calculation, and duplicate marking. Note this nf-core module requires files to be copied into the working directory and not symlinked.

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

reads:file

fastq.gz files

*.fastq.gz

meta2:map

Groovy Map containing fasta information

fasta:file

reference fasta file - must be unzipped

*.fasta

meta3:map

Groovy Map containing index information

index:file

reference BWA index

*.{amb,ann,bwt,pac,sa}

meta4:map

Groovy Map containing index information

interval_file:file

(optional) file(s) containing genomic intervals for use in base quality score recalibration (BQSR)

*.{bed,interval_list,picard,list,intervals}

known_sites:file

(optional) known sites file(s) for calculating BQSR. markdups must be true to perform BQSR.

*.vcf.gz

Output

name:type
description
pattern

bam

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.bam:file

Sorted BAM file

*.bam

bai

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

*.bai:file

index corresponding to sorted BAM file

*.bai

bqsr_table

meta:map

Groovy Map containing sample information e.g. [ id:‘test’]

*.table:file

(optional) table from base quality score recalibration calculation, to be used with parabricks/applybqsr

*.table

versions

versions.yml:file

File containing software versions

versions.yml

qc_metrics

qc_metrics:directory

(optional) optional directory of qc metrics

qc_metrics

duplicate_metrics

duplicate-metrics.txt:file

(optional) metrics calculated from marking duplcates in the bam file

*-duplicate-metrics.txt

Tools

parabricks
custom

NVIDIA Clara Parabricks GPU-accelerated genomics tools