Description

Takes one or multiple sequencing chips of an amplified library as HiFi reads and marks or removes duplicates.

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

input

:file

Sequencing reads in BAM, FASTQ, or FASTA format.

*.{bam,f*a,/.*f.*\.gz/}

Output

name:type
description
pattern

markduped

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

${prefix}.${suffix}

:file

Markduplicated sequencing reads in the same format as the input file.

*.{bam,f*a,/.*f.*\.gz/}

dupfile

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

${dupfile_name}

:file

(Optional) File listing duplicate reads (Specify by –dup-file).

*.{bam,f*a,/.*f.*\.gz/}

log

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

*.pbmarkdup.log

:file

Log file generated by pbmarkdup (if –log-level is specified).

*.pbmarkdup.log

versions_pbmarkdup

${task.process}

:string

Name of the process

pbmarkdup

:string

The name of the tool

pbmarkdup --version | cut -d' ' -f2

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

Name of the process

pbmarkdup

:string

The name of the tool

pbmarkdup --version | cut -d' ' -f2

:eval

The expression to obtain the version of the tool

Tools

pbmarkdup
BSD-3-Clause

pbmarkdup identifies and marks duplicate reads in PacBio HiFi (CCS) data. It clusters highly similar CCS reads to detect PCR duplicates and flags them in the output files (BAM,FASTQ,FASTA) (duplicate bit 0x400), optionally removing duplicates. (duplicate bit 0x400), optionally removing duplicates.