Description

Runs PEKA CLIP peak k-mer analysis

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

peaks (file)

BED file of peak regions

*.{bed,bed.gz}

BED file of crosslinks

*.{bed,bed.gz}

fasta (file)

Genome reference sequence used

*.{fa,fasta}

fai (file)

FAI file corresponding to the reference sequence

*.{fai}

gtf (file)

A segmented GTF used to annotate peaks

*.{gtf}

Output

Name (Type)
Description
Pattern

cluster (file)

TSV file of summed occurrence distributions of k-mers within defined clusters

*.tsv

distribution (file)

TSV file with calculated PEKA score and occurrence distribution for all possible k-mers

*.tsv

pdf (file)

PDF file with graphs showing k-mer occurrence distributions around thresholded crosslink sites

*.pdf

versions (file)

File containing software versions

versions.yml

Tools

peka
GPL v3

Positionally-enriched k-mer analysis (PEKA) is a software package for identifying enriched protein-RNA binding motifs from CLIP datasets