Description

Collect multiple metrics from a BAM file

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bam{:bash}

:file

SAM/BAM/CRAM file

*.{sam,bam,cram}

bai{:bash}

:file

Optional SAM/BAM/CRAM file index

*.{sai,bai,crai}

meta2{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘genome’]

fasta{:bash}

:file

Genome fasta file

meta3{:bash}

:map

Groovy Map containing reference information e.g. [ id:‘genome’]

fai{:bash}

:file

Index of FASTA file. Only needed when fasta is supplied.

*.fai

Output

name:type
description
pattern

metrics{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*_metrics{:bash}

:file

Alignment metrics files generated by picard

*_{metrics}

pdf{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.pdf{:bash}

:file

PDF plots of metrics

*.{pdf}

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

picard
MIT

A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.