Description

Collect metrics from a RNAseq BAM file

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

, strandedness
]

bam (file)

BAM/SAM file

*.{bam,sam}

ref_flat (file)

Genome ref_flat file

gene_pred (file)

genome gene_pred file as an alternative to the ref_flat file

fasta (file)

Genome fasta file

rrna_intervals (file)

Interval file of ribosomal RNA regions

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

metrics (file)

RNA alignment metrics files generated by picard

*.rna_metrics

pdf (file)

Plot normalized position vs. coverage in a pdf file generated by picard

versions (file)

File containing software versions

versions.yml

Tools

picard
MIT

A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.