Description

Collect metrics from a RNAseq BAM file

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false, strandedness:true ]

bam{:bash}

:file

BAM/SAM file

*.{bam,sam}

ref_flat{:bash}

:file

Genome ref_flat file

fasta{:bash}

:file

Genome fasta file

rrna_intervals{:bash}

:file

Interval file of ribosomal RNA regions

Output

name:type
description
pattern

metrics{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.rna_metrics{:bash}

:file

RNA alignment metrics files generated by picard

*.rna_metrics

pdf{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.pdf{:bash}

:file

Plot normalized position vs. coverage in a pdf file generated by picard

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

picard
MIT

A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.