Description

Collect metrics from a RNAseq BAM file

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false, strandedness:true ]

bam

:file

BAM/SAM file

*.{bam,sam}

ref_flat

:file

Genome ref_flat file

fasta

:file

Genome fasta file

rrna_intervals

:file

Interval file of ribosomal RNA regions

Output

name:type
description
pattern

metrics

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.rna_metrics

:file

RNA alignment metrics files generated by picard

*.rna_metrics

pdf

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.pdf

:file

Plot normalized position vs. coverage in a pdf file generated by picard

versions_picard

${task.process}

:string

The process the versions were collected from

picard

:string

The tool name

picard CollectRnaSeqMetrics --version 2>&1 | sed -n 's/.*Version://p'

:string

The command used to generate the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The process the versions were collected from

picard

:string

The tool name

picard CollectRnaSeqMetrics --version 2>&1 | sed -n 's/.*Version://p'

:string

The command used to generate the version of the tool

Tools

picard
MIT

A set of command line tools (in Java) for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF.