Description

Quality control of riboseq bam data

Input

name:type
description
pattern

meta

:map

Groovy Map containing riboseq sample information e.g. `[ id:‘sample1’, single_end:false ]

bam_ribo

:file

Sorted riboseq BAM file(s)

*.{bam}

bai_ribo

:file

Index for sorted riboseq bam file(s)

*.{bai}

meta2

:map

Groovy Map containing TI-Seq sample information e.g. [ id:'sample1', single_end:false ]

bam_ti

:file

Sorted TI-Seq BAM file(s)

*.{bam}

bai_ti

:file

Index for sorted TI-Seq BAM file(s)

*.{bai}

meta3

:map

Groovy Map containing reference information e.g. [ id:'Ensembl human v.111' ]

fasta

:file

Fasta-format sequence file for reference sequences used in the bam file

*.gtf

gtf

:file

GTF-format annotation file for reference sequences used in the bam file

*.gtf

meta4

:map

Groovy Map containing information on candidate ORFs

candidate_orfs

:file

3-column (transIDstarttstop) candidate ORFs file

*.txt

meta5

:map

Groovy Map containing information on riboseq P-site offset parameter files

para_ribo

:file

Input P-site offset parameter files for riboseq bam files

*.py

meta6

:map

Groovy Map containing information on TI-seq P-site offset parameter files

para_ti

:file

Input P-site offset parameter files for TI-seq bam files

*.py

meta7

:map

Groovy Map containing reference information for the secondary annotation file

reference_gtf

:file

Optional secondary GTF annotation passed to ribotish as -a <reference-annotation> (e.g. a MANE/RefSeq overlay applied on top of the primary GTF). Pass [] to omit.

*.gtf

Output

name:type
description
pattern

predictions

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*_pred.txt

:file

txt file all possible ORF results that fit the thresholds

*.{txt}

all

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*_all.txt

:file

txt file similar to the predictions but do not use FDR (q-value) cutoff

*.{txt}

transprofile

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*_transprofile.py

:file

Output RPF P-site profile for each transcript. The profile data is in python dict format, recording non-zero read counts at different positions on transcript.

*.{py}

versions_ribotish

${task.process}

:string

The name of the process

ribotish

:string

The name of the tool

ribotish --version | sed 's/ribotish //'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

ribotish

:string

The name of the tool

ribotish --version | sed 's/ribotish //'

:eval

The expression to obtain the version of the tool

Tools

ribotish
GPL v3

Ribo TIS Hunter (Ribo-TISH) identifies translation activities using ribosome profiling data.