Description

Quality control of riboseq bam data

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

bam{:bash}

:file

Sorted BAM/CRAM/SAM file

*.{bam}

bai{:bash}

:file

Index for sorted BAM/CRAM/SAM file

*.{bai}

meta2{:bash}

:map

Groovy Map containing reference information e.g. [ id:'Ensembl human v.111' ]

gtf{:bash}

:file

GTF-format annotation file for reference sequences used in the bam file

*.gtf

Output

name:type
description
pattern

distribution{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.txt{:bash}

:file

file containing distribution

*.{txt}

pdf{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.pdf{:bash}

:file

Pdf figure file is plot of all the distributions and illustration of quality and P-site offset

*.{pdf}

offset{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.para.py{:bash}

:file

This file saves P-site offsets for different reads lengths in python code dict format, and can be used in further analysis

*.{para.py}

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

ribotish
GPL v3

Ribo TIS Hunter (Ribo-TISH) identifies translation activities using ribosome profiling data.