Description

Calculate expression with RSEM

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

Input reads for quantification (FASTQ files or BAM file for —alignments mode)

*.{fastq.gz,bam}

index

:file

RSEM index

rsem/*

Output

name:type
description
pattern

counts_gene

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.genes.results

:file

Expression counts on gene level

*.genes.results

counts_transcript

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.isoforms.results

:file

Expression counts on transcript level

*.isoforms.results

stat

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.stat

:file

RSEM statistics

*.stat

logs

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.log

:file

RSEM logs

*.log

versions_rsem

${task.process}

:string

The name of the process

rsem

:string

The name of the tool

rsem-calculate-expression --version | sed 's/Current version: RSEM v//'

:eval

The expression to obtain the version of the tool

versions_star

${task.process}

:string

The name of the process

star

:string

The name of the tool

STAR --version | sed -e "s/STAR_//g"

:eval

The expression to obtain the version of the tool

bam_star

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.STAR.genome.bam

:file

BAM file generated by STAR (optional)

*.STAR.genome.bam

bam_genome

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

${prefix}.genome.bam

:file

Genome BAM file (optional)

*.genome.bam

bam_transcript

meta

:map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

${prefix}.transcript.bam

:file

Transcript BAM file (optional)

*.transcript.bam

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

rsem

:string

The name of the tool

rsem-calculate-expression --version | sed 's/Current version: RSEM v//'

:eval

The expression to obtain the version of the tool

${task.process}

:string

The name of the process

star

:string

The name of the tool

STAR --version | sed -e "s/STAR_//g"

:eval

The expression to obtain the version of the tool

Tools

rseqc
GPL-3.0-or-later

RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome