Description

Converts a SAM/BAM/CRAM file to FASTQ

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

input{:bash}

:file

BAM/CRAM/SAM file

*.{bam,cram,sam}

interleave{:bash}

:boolean

Set true for interleaved fastq file

Output

name:type
description
pattern

fastq{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*_{1,2}.fastq.gz{:bash}

:file

Compressed FASTQ file(s) with reads with either the READ1 or READ2 flag set in separate files.

*_{1,2}.fastq.gz

interleaved{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*_interleaved.fastq{:bash}

:file

Compressed FASTQ file with reads with either the READ1 or READ2 flag set in a combined file. Needs collated input file.

*_interleaved.fastq.gz

singleton{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*_singleton.fastq.gz{:bash}

:file

Compressed FASTQ file with singleton reads

*_singleton.fastq.gz

other{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*_other.fastq.gz{:bash}

:file

Compressed FASTQ file with reads with either both READ1 and READ2 flags set or unset

*_other.fastq.gz

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

samtools
MIT

SAMtools is a set of utilities for interacting with and post-processing short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA.