Description

Call peaks using SEACR on sequenced reads in bedgraph format

Input

name:type
description
pattern

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bedgraph{:bash}

:file

The target bedgraph file from which the peaks will be calculated.

ctrlbedgraph{:bash}

:file

Control (IgG) data bedgraph file to generate an empirical threshold for peak calling.

threshold{:bash}

:integer

Threshold value used to call peaks if the ctrlbedgraph input is set to []. Set to 1 if using a control bedgraph

Output

name:type
description
pattern

bed{:bash}

meta{:bash}

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.bed{:bash}

:file

Bed file containing the calculated peaks.

*.bed

versions{:bash}

versions.yml{:bash}

:file

File containing software versions

versions.yml

Tools

seacr
GPL-2.0-only

SEACR is intended to call peaks and enriched regions from sparse CUT&RUN or chromatin profiling data in which background is dominated by "zeroes" (i.e. regions with no read coverage).