Description

A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

FASTA or FASTQ files

*.{fa,fasta,fq,fastq,fq.gz,fastq.gz}

meta2

:map

Groovy Map containing genome information e.g. [ id:‘genome’ ]

fasta

:file

Reference genome FASTA file used to construct Segemehl

*.{fa,fasta}

index

:file

Segemehl Index file from SEGEMEHL_INDEX

*.idx

Output

name:type
description
pattern

alignment

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}/${prefix}.${suffix}

:file

File containing genomic alignments in SAM format (please add “-b” flag to task.ext.args for BAM)

*.{sam,bam}

trans_alignments

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}/${prefix}.trns.txt

:file

Custom text file containing all single split alignments predicted to be in trans (optional, only if -S flag is set in task.ext.args)

*.trns.txt

multi_bed

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}/${prefix}.mult.bed

:file

Bed file containing all splice events predicted in the split read alignments. (optional, only if -S flag is set in task.ext.args)

*.mult.bed

single_bed

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}/${prefix}.sngl.bed

:file

Bed file containing all single splice events predicted in the split read alignments. (optional, only if -S flag is set in task.ext.args)

*.sngl.bed

versions_segemehl

${task.process}

:string

The name of the process

segemehl

:string

The name of the tool

segemehl.x 2>&1 | sed '/^ [0-9]\.[0-9\.]*/!d;s/^ //;s/ .*//'

:eval

The expression to obtain the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The name of the process

segemehl

:string

The name of the tool

segemehl.x 2>&1 | sed '/^ [0-9]\.[0-9\.]*/!d;s/^ //;s/ .*//'

:eval

The expression to obtain the version of the tool

Tools

segemehl
GPL v3

A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection