Description

Collects multiple quality metrics from a bam file

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

bam

:file

Sorted BAM/CRAM/SAM file

*.{bam,cram,sam}

bai

:file

Index of th sorted BAM/CRAM/SAM file

*.{bai,crai,sai}

meta2

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

fasta

:file

Genome fasta file

*.{fa,fasta}

meta3

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

fai

:file

Index of the genome fasta file

*.fai

plot_results

:boolean

Boolean to determine whether plots should be generated

true or false

Output

name:type
description
pattern

mq_metrics

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*mq_metrics.txt

:file

File containing the information about mean base quality score for each sequencing cycle

*.txt

qd_metrics

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*qd_metrics.txt

:file

File containing the information about number of bases with a specific base quality score

*.txt

gc_summary

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*gc_summary.txt

:file

File containing the information about GC bias in the reference and the sample

*.txt

gc_metrics

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*gc_metrics.txt

:file

File containing the information about GC bias in the reference and the sample

*.txt

aln_metrics

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*aln_metrics.txt

:file

File containing the statistics about the alignment of the reads

*.txt

is_metrics

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*is_metrics.txt

:file

File containing the information about statistical distribution of insert sizes

*.txt

mq_plot

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*mq_metrics.pdf

:file

PDF containing plot of mean base quality scores

*.pdf

qd_plot

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*qd_metrics.pdf

:file

PDF containing plot of specific base quality score

*.pdf

is_plot

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*is_metrics.pdf

:file

PDF containing plot of insert sizes

*.pdf

gc_plot

meta

:map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*gc_metrics.pdf

:file

PDF containing plot of GC bias

*.pdf

versions_sentieon

${task.process}

:string

The process the versions were collected from

sentieon

:string

The tool name

sentieon driver --version | sed "s/.*-//g"

:string

The command used to generate the version of the tool

Topics

name:type
description
pattern

versions

${task.process}

:string

The process the versions were collected from

sentieon

:string

The tool name

sentieon driver --version | sed "s/.*-//g"

:string

The command used to generate the version of the tool

Tools

sentieon

Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system.