modules/sentieon_datametrics

Collects multiple quality metrics from a bam file

metricsbamsentieon

https://github.com/nf-core/modules/[...]/modules/nf-core/sentieon/datametrics

Description

Collects multiple quality metrics from a bam file

Input

name:type

description

pattern

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`bam:file`

Sorted BAM/CRAM/SAM file

*.{bam,cram,sam}

`bai:file`

Index of th sorted BAM/CRAM/SAM file

*.{bai,crai,sai}

`meta2:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`fasta:file`

Genome fasta file

*.{fa,fasta}

`meta3:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`fai:file`

Index of the genome fasta file

*.fai

`plot_results:boolean`

Boolean to determine whether plots should be generated

true or false

Output

name:type

description

pattern

`mq_metrics`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*mq_metrics.txt:file`

File containing the information about mean base quality score for each sequencing cycle

*.txt

`qd_metrics`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*qd_metrics.txt:file`

File containing the information about number of bases with a specific base quality score

*.txt

`gc_summary`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*gc_summary.txt:file`

File containing the information about GC bias in the reference and the sample

*.txt

`gc_metrics`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*gc_metrics.txt:file`

File containing the information about GC bias in the reference and the sample

*.txt

`aln_metrics`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*aln_metrics.txt:file`

File containing the statistics about the alignment of the reads

*.txt

`is_metrics`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*is_metrics.txt:file`

File containing the information about statistical distribution of insert sizes

*.txt

`mq_plot`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*mq_metrics.pdf:file`

PDF containing plot of mean base quality scores

*.pdf

`qd_plot`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*qd_metrics.pdf:file`

PDF containing plot of specific base quality score

*.pdf

`is_plot`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*is_metrics.pdf:file`

PDF containing plot of insert sizes

*.pdf

`gc_plot`

`meta:map`

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

`*gc_metrics.pdf:file`

PDF containing plot of GC bias

*.pdf

`versions`

`versions.yml:file`

File containing software versions

versions.yml

Tools

sentieon

Sentieon® provides complete solutions for secondary DNA/RNA analysis for a variety of sequencing platforms, including short and long reads. Our software improves upon BWA, STAR, Minimap2, GATK, HaplotypeCaller, Mutect, and Mutect2 based pipelines and is deployable on any generic-CPU-based computing system.

sentieon.com