Description

Indexing of transcriptome for gene expression quantification using SimpleAF

Input

name:type
description
pattern

meta:map

Groovy Map containing information on genome_fasta

genome_fasta:file

FASTA file containing the genome sequence

meta2:map

Groovy Map containing information on genome_gtf

genome_gtf:file

GTF file containing transcript annotations. Optional if transcript FASTA file is provided.

meta3:map

Groovy Map containing information on transcript_fasta

transcript_fasta:file

FASTA file containing the transcript sequences. Optional if transcript GTF file is provided.

Output

name:type
description
pattern

index

meta:map

Groovy Map containing information on genome_fasta or transcript_fasta (whichever was used)

${prefix}/index:directory

Folder containing the Salmon index files

salmon/index

transcript_tsv

meta:map

Groovy Map containing information on genome_fasta or transcript_fasta (whichever was used)

${prefix}/ref/t2g_3col.tsv:file

Transcript-to-gene mapping file in 3-column TSV format

salmon/ref/*_t2g_3col.tsv

salmon

meta:map

Groovy Map containing information on genome_fasta or transcript_fasta (whichever was used)

${prefix}:directory

Folder containing the Salmon files

salmon

versions

versions.yml:file

File containing software versions

versions.yml

Tools

simpleaf
BSD-3-Clause

SimpleAF is a tool for quantification of gene expression from RNA-seq data