Description

simpleaf is a program to simplify and customize the running and configuration of single-cell processing with alevin-fry.

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

reads (file)

List of input FastQ files for paired-end data.
Reads should be grouped by pairs.

meta2 (map)

Groovy Map containing index information

index (directory)

Folder containing the index files

meta3 (map)

Groovy Map containing txp2gene information

txp2gene (file)

File mapping transcripts to genes.

chemistry (string)

Chemistry used for library preparation. It can be a string describing
the specific chemistry or the geometry of the barcode, UMI, and
mappable read. For example, “10xv2” and “10xv3” will apply the
appropriate settings for 10x Chromium v2 and v3 protocols,
respectively. Alternatively, you can provide a general geometry string
if your chemistry is not pre-registered. For example, instead of
”10xv2”, you could use “1{b[16]u[10]x:}2{r:}”, or instead of “10xv3”,
you could use “1{b[16]u[12]x:}2{r:}“.

meta4 (map)

Groovy Map containing whitelist information

whitelist (file)

Whitelist file containing valid cell barcodes. Optional.

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

alevin_results (directory)

Folder containing the Alevin results for a specific sample

*

versions (file)

File containing software versions

versions.yml

Tools

simpleaf
BSD-3-Clause

SimpleAF is a tool for quantification of gene expression from RNA-seq data