Description

Module to build a filtered GTF needed by the 10x Genomics Space Ranger tool. Uses the spaceranger mkgtf command.

Input

name:type
description
pattern

gtf:file

The reference GTF transcriptome file

*.gtf

Output

name:type
description
pattern

gtf

*.gtf:directory

The filtered GTF transcriptome file

*.filtered.gtf

versions

versions.yml:file

File containing software version

versions.yml

Tools

spaceranger
10x Genomics EULA

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Space Ranger is a set of analysis pipelines that process Visium Spatial Gene Expression data with brightfield and fluorescence microscope images. Space Ranger allows users to map the whole transcriptome in formalin fixed paraffin embedded (FFPE) and fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research. Space Ranger provides pipelines for end to end analysis of Visium Spatial Gene Expression experiments.