Description

Short Read Sequence Typing for Bacterial Pathogens is a program designed to take Illumina sequence data, a MLST database and/or a database of gene sequences (e.g. resistance genes, virulence genes, etc) and report the presence of STs and/or reference genes.

Input

Name (Type)
Description
Pattern

meta (map0.2.0-4)

Groovy Map containing sample information
id: should be the identification number or sample name
single_end: should be true for single end data and false for paired in data
db: should be either ‘gene’ to use the —gene_db option or “mlst” to use the —mlst_db option
e.g. [ id:‘sample’, single_end

, db:‘gene’]

fasta (file)

gzipped fasta file. If files are NOT in
MiSeq format sample_S1_L001_R1_001.fastq.gz uses —forward and —reverse parameters; otherwise
default is _1, i.e. expect forward reads as sample_1.fastq.gz).

*.fastq.gz

db (file)

Database in FASTA format

*.fasta

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘sample’, single_end

]

versions (file)

File containing software versions

versions.yml

txt (file)

A detailed report, with one row per gene per sample described here github.com/katholt/srst2#gene-typing

*_fullgenes_*_results.txt

txt (file)

A tabulated summary report of samples x genes.

*_genes_*_results.txt

txt (file)

A tabulated summary report of mlst subtyping.

*_mlst_*_results.txt

bam (file)

Sorted BAM file

*.sorted.bam

pileup (file)

SAMtools pileup file

*.pileup