Description

Run TRUST4 on RNA-seq data

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:'sample1', single_end:false ]

bam (file)

BAM file from bulk or single-cell RNA-seq data

*.bam

reads (file)

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively

fasta (file)

Path to the fasta file coordinate and sequence of V/D/J/C genes

ref (file)

Path to detailed V/D/J/C gene reference file, such as from IMGT database.

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:'sample1', single_end:false ]

tsv (file)

tsv files created by TRUST4

*.tsv

airr_tsv (file)

TRUST4 results in AIRR format

*_airr.tsv

report_tsv (file)

TRUST4 report in tsv format

*_report.tsv

fasta (file)

Fasta files created by TRUST4

*.fa

out (file)

Further report files

*.out

fq (file)

Fastq files created by TRUST4

*.fq

versions (file)

File containing software versions

versions.yml

Tools

trust4
GPL v3

TCR and BCR assembly from bulk or single-cell RNA-seq data