Description

Import transcript-level abundances and estimated counts for gene-level analysis packages

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing information related to the experiment as a whole
e.g. [ id:'SRP123456' ]

quants (directory)

Paths to subdirectories corresponding to sample-wise runs of Salmon or Kallisto

meta2 (map)

Groovy Map containing reference information related to the species
reference e.g. [ id:'yeast' ]

tx2gene (file)

A transcript to gene mapping table such as those generated by custom/tx2gene

*.{csv,tsv}

meta3 (map)

Groovy Map containing information related to the experiment as a whole
e.g. [ id:'SRP123456' ]

coldata (file)

Optional ‘coldata’ file equivalent to a sample sheet where the first
column corresponds to the sample names (directory names in the input
salmon/ kallisto results)

*.{csv,tsv}

quant_type (string)

Quantification type, ‘kallisto’ or ‘salmon’

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing information related to the experiment as a whole
e.g. [ id:'SRP123456' ]

tpm_gene (file)

Abundance (TPM) values derived from tximport output after
summarizeToGene(), without a ‘countsFromAbundance’ specification

*gene_tpm.tsv

counts_gene (file)

Count values derived from tximport output after
summarizeToGene(), without a ‘countsFromAbundance’ specification

*gene_counts.tsv

counts_gene_length_scaled (file)

Count values derived from tximport output after summarizeToGene(), with
a ‘countsFromAbundance’ specification of ‘lengthScaledTPM’

*gene_counts_length_scaled.tsv

counts_gene_scaled (file)

Count values derived from tximport output after summarizeToGene(), with
a ‘countsFromAbundance’ specification of ‘scaledTPM’

*gene_counts_scaled.tsv

lengths_gene (file)

Length values derived from tximport output after summarizeToGene(),
without a ‘countsFromAbundance’ specification

*gene_lengths.tsv

tpm_transcript (file)

Abundance (TPM) values derived from tximport output without
summarizeToGene(), without a ‘countsFromAbundance’ specification

*transcript_tpm.tsv

counts_transcript (file)

Count values derived from tximport output without
summarizeToGene(), without a ‘countsFromAbundance’ specification

*transcript_counts.tsv

lengths_transcript (file)

Length values derived from tximport output without summarizeToGene(),
without a ‘countsFromAbundance’ specification

*gene_lengths.tsv

versions (file)

File containing software versions

versions.yml