Description

Deduplicate reads based on the mapping co-ordinate and the UMI attached to the read.

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

bam (file)

BAM file containing reads to be deduplicated via UMIs.

*.{bam}

bai (file)

BAM index files corresponding to the input BAM file. Optionally can be skipped using [] when using FastQ input.

*.{bai}

mode (string)

Selects the mode of Umicollapse - either fastq or bam need to be provided.

{fastq,bam}

Output

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

bam (file)

BAM file with deduplicated UMIs.

*.{bam}

log (file)

A log file with the deduplication statistics.

*_{UMICollapse.log}

versions (file)

File containing software versions

versions.yml

Tools

umicollapse
MIT

UMICollapse contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs).