Description

Group reads based on their UMI and mapping coordinates

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

bam

:file

BAM file containing reads to be deduplicated via UMIs.

*.{bam}

bai

:file

BAM index files corresponding to the input BAM file.

*.{bai}

create_bam

:boolean

Whether or not to create a read group tagged BAM file.

get_group_info

:boolean

Whether or not to generate the flatfile describing the read groups, see docs for complete info of all columns

Output

name:type
description
pattern

log

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.log

:file

File with logging information

*.{log}

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

${prefix}.bam

:file

a read group tagged BAM file.

${prefix}.{bam}

tsv

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*.tsv

:file

Flatfile describing the read groups, see docs for complete info of all columns

*.{tsv}

versions

versions.yml

:file

File containing software versions

versions.yml

Tools

umi_tools

UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes