Description

Use vireo to perform donor deconvolution for multiplexed scRNA-seq data

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:'sample1']

cell_data:file

The cell genotype file in VCF format or cellSNP folder with sparse matrices.

*.vcf|*/

n_donor:integer

Number of donors to demultiplex.

donor_file:file

The optional donor genotype file in VCF format.

*.vcf

vartrix_data:file

The optional cell genotype files in vartrix outputs.

Output

name:type
description
pattern

summary

meta:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

*_summary.tsv:file

Summary tsv file of deconvolution result.

*_summary.tsv

donor_ids

meta:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

*_donor_ids.tsv:file

Donor assignment with detailed statistics.

*_donor_ids.tsv

prob_singlets

meta:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

*_prob_singlet.tsv.gz:file

contains probability of classifing singlets

*_prob_singlet.tsv.gz

prob_doublets

meta:map

Groovy Map containing sample information e.g. [ id:'sample1' ]

*_prob_doublet.tsv.gz:file

contains probability of classifing doublets

*_prob_doublet.tsv.gz

versions

versions.yml:file

File containing software versions

versions.yml

Tools

vireo
Apache-2.0

vireoSNP - donor deconvolution for multiplexed scRNA-seq data