Description

Read QC, UMI extraction and trimming

Input

name
description
pattern

meta

Groovy Map containing sample information
e.g. [ id:‘test’ ]

reads

List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.

skip_fastqc

Skip fastqc process

with_umi

With or without umi detection

skip_umi_extract

With or without umi extrection

umi_discard_read

Discard R1 / R2 if required

skip_trimming

Allows to skip FastP execution

adapter_fasta

Fasta file of adapter sequences

save_trimmed_fail

Save trimmed fastqs of failed samples

save_merged

Save merged fastqs

min_trimmed_reads

Inputs with fewer than this reads will be filtered out of the “reads” output channel

Output

name
description
pattern

meta

Groovy Map containing sample information
e.g. [ id:‘test’ ]

reads

Extracted FASTQ files. | For single-end reads, pattern is ${prefix}.umi_extract.fastq.gz. | For paired-end reads, pattern is ${prefix}.umi_extract_{1,2}.fastq.gz.

*.{fastq.gz}

fastqc_html

FastQC report

*_{fastqc.html}

fastqc_zip

FastQC report archive

*_{fastqc.zip}

log

Logfile for umi_tools

*.{log}

trim_json

FastP Trimming report

*.{fastp.json}

trim_html

FastP Trimming report

*.{fastp.html}

log

Logfile FastP

*.{fastp.log}

trim_reads_fail

Trimmed fastq files failing QC

*.{fastq.gz}

trim_reads_merged

Trimmed and merged fastq files

*.{fastq.gz}

trim_read_count

Number of reads after trimming

fastqc_trim_html

FastQC report

*_{fastqc.html}

fastqc_trim_zip

FastQC report archive

*_{fastqc.zip}

adapter_seq

Adapter Sequence found in read1

versions

File containing software versions

versions.yml